|Year : 2020 | Volume
| Issue : 2 | Page : 68-76
Repetitively hypoxic preconditioning attenuates ischemia/reperfusion-induced liver dysfunction through upregulation of hypoxia-induced factor-1 alpha-dependent mitochondrial Bcl-xl in rat
Pei-Lei Chou1, Kuo-Hsin Chen2, Tzu-Ching Chang1, Chiang-Ting Chien1
1 School of Life Science, National Taiwan Normal University, Taipei, Taiwan
2 Department of Surgery, Division of General Surgery, Far-Eastern Memorial Hospital; Department of Electrical Engineering, Yuan Ze University, Taoyuan City, Taiwan
|Date of Submission||14-Oct-2019|
|Date of Acceptance||13-Feb-2020|
|Date of Web Publication||27-Apr-2020|
Prof. Chiang-Ting Chien
No. 88, Sec. 4, Tingzhou Road, School of Life Science, National Taiwan Normal University, Taipei 11677
Prof. Tzu-Ching Chang
No. 88, Sec. 4, Tingzhou Road, School of Life Science, National Taiwan Normal University, Taipei 11677
Source of Support: This work was supported in part by the National Science
Council of the Republic of China (NSC96.2320.B.002.007
and NSC96.2221.E.002.256.MY3)., Conflict of Interest: None
Repetitive hypoxic preconditioning (HP) enforces protective effects to subsequently severe hypoxic/ischemic stress. We hypothesized that HP may provide protection against ischemia/reperfusion (I/R) injury in rat livers via hypoxia-induced factor-1 alpha (HIF-1α)/reactive oxygen species (ROS)-dependent defensive mechanisms. Female Wistar rats were exposed to hypoxia (15 h/day) in a hypobaric hypoxic chamber (5500 m) for HP induction, whereas the others were kept in sea level. These rats were subjected to 45 min of hepatic ischemia by portal vein occlusion followed by 6 h of reperfusion. We evaluated HIF-1α in nuclear extracts, MnSOD, CuZnSOD, catalase, Bad/Bcl-xL/caspase 3/poly-(ADP-ribose)-polymerase (PARP), mitochondrial Bcl-xL, and cytosolic cytochrome C expression with Western blot and nitroblue tetrazolium/3-nitrotyrosine stain. Kupffer cell infiltration and terminal deoxynucleotidyl transferase-mediated nick-end labeling method apoptosis were determined by immunocytochemistry. The ROS value from liver surface and bile was detected by an ultrasensitive chemiluminescence–amplification method. Hepatic function was assessed with plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. HP increased nuclear translocation of HIF-1α and enhanced Bcl-xL, MnSOD, CuZnSOD, and catalase protein expression in a time-dependent manner. The response of HP enhanced hepatic HIF-1α, and Bcl-xL expression was abrogated by a HIF-1α inhibitor YC-1. Hepatic I/R increased ROS levels, myeloperoxidase activity, Kupffer cell infiltration, ALT and AST levels associated with the enhancement of cytosolic Bad translocation to mitochondria, release of cytochrome C to cytosol, and activation of caspase 3/PARP-mediated apoptosis. HP significantly ameliorated hepatic I/R-enhanced oxidative stress, apoptosis, and mitochondrial and hepatic dysfunction. In summary, HP enhances HIF-1α/ROS-dependent cascades to upregulate mitochondrial Bcl-xL protein expression and to confer protection against I/R injury in the livers.
Keywords: Apoptosis, Bcl-xL, hypoxia-inducible factor-1α, hypoxic preconditioning, reactive oxygen species
|How to cite this article:|
Chou PL, Chen KH, Chang TC, Chien CT. Repetitively hypoxic preconditioning attenuates ischemia/reperfusion-induced liver dysfunction through upregulation of hypoxia-induced factor-1 alpha-dependent mitochondrial Bcl-xl in rat. Chin J Physiol 2020;63:68-76
|How to cite this URL:|
Chou PL, Chen KH, Chang TC, Chien CT. Repetitively hypoxic preconditioning attenuates ischemia/reperfusion-induced liver dysfunction through upregulation of hypoxia-induced factor-1 alpha-dependent mitochondrial Bcl-xl in rat. Chin J Physiol [serial online] 2020 [cited 2020 Aug 15];63:68-76. Available from: http://www.cjphysiology.org/text.asp?2020/63/2/68/283348
Pei-Lei Chou and Kuo-Hsin Chen contributed equally to this work.
| Introduction|| |
Under hypoxic/ischemic conditions, severe hypoxia/ischemia leads to cell and tissue impairment. However, the tolerance resistant to hypoxia/ischemia can be achieved by hypoxic preconditioning (HP), ischemic preconditioning,, and hyperthermic preconditioning. The mechanisms of these preconditioning methods possibly involved the release of adenosine, prostacyclin, reactive oxygen species (ROS), and Akt signaling transmitters.,,, The single hypoxic or ischemic preconditioning technique exerts mitochondrial-independent and mitochondrial-dependent protective pathways to the following ischemic damage.,,,, Furthermore, repetitive HP can afford efficiently and significantly longer protection than single preconditioning.,
The mitochondrion is an important cellular component responsible for regulating energy, oxidative metabolism, and adaption to hypoxic/ischemic condition. Mitochondrial dysfunction following ischemia/reperfusion (I/R) injury can induce apoptotic cell death, since the mitochondrial apoptogenic factor, cytochrome C, is released into the cytoplasm to activate caspase-3-mediated apoptosis. Cytochrome C leakage to cytosol can be triggered by pro-apoptogenic Bax/Bad, and inhibited by Bcl-2/Bcl-xL and heat shock protein 70 (HSP70). On the other hand, hypoxia-inducible factor-1 (HIF-1), consisting of HIF-1β and HIF-1α subunits, enhances protective genes, including Bcl-2, Bcl-xL, erythropoietin, HSP70, and vascular endothelial growth factor (VEGF), and protects against oxidative injury. HIF-1α activation could also downregulate the proapoptotic Bax/Bid/Bad expression., Under hypoxia, HIF-1α is upregulated to high levels at 3–6 h and decreased thereafter. Our previous study confirmed that HP reduced renal I/R injury through HIF-1-mediated Bcl-2 or HSP70 protective pathways. HP can promote the survival of cardiac progenitor cells because HP time dependently enhances the Pim-1 kinase to inhibit proapoptotic factors and restores mitochondria Bcl-2/Bcl-xL to decrease mitochondrial injury. In addition, long-term cycles of hypoxia/normoxia conditioning decreased oxidative stress and liver dysfunction but increased the antioxidant superoxide dismutase levels and improved mitochondrial respiratory function in the rat liver. We hypothesized that HP might induce mitochondrial protection to reduce hepatic I/R injury-induced oxidative injury via HIF-1α-dependent pathway. In clinical trials, the expression of the cytoprotective protein heme oxygenase-1 is significantly upregulated in the liver within minutes of intermittent clamping of the porta hepatis, which is commonly performed during liver surgery to reduce blood loss and has been reported to precondition livers, resulting in improved outcome after liver surgery in humans. Large population studies have displayed that living at higher altitudes with lower oxygen exposure is associated with the reduced cardiovascular disease mortality by the role of HIF-1α in increasing anti-inflammatory IL-10 transactivation.
In this study, we address the possible role of HIF-1α and several antioxidant and antiapoptotic proteins in HP-induced protective mechanism in rat livers.
| Materials and Methods|| |
Repetitive hypoxic preconditioning
Female Wistar rats (200–250 g) were purchased from BioLASCO Taiwan Co. Ltd. (Taipei) and housed at the Experimental Animal Center of National Taiwan University with constant temperature and consistent light cycle (light from 07:00 to 18:00). The animal care and experimental protocols were approved by the National Taiwan University of Public Health Institutional Animal Care and Use Committee (IACUC ethic code No: 20040321) and were in accordance with the guidelines of the Ministry of Science and Technology of the Republic of China. The HP induction was performed as described previously. In brief, the animals were placed in a simulated high altitude chamber (HP) 15 h/day, whereas age-matched control animals were maintained at sea level (normoxic group, SL). We selected the 380 torr (5500 m) for hypoxic induction, because, at this altitude, these rats can well adapt. The body weight of the animals was determined every week. Food and water were provided ad libitum.
YC-1 on hypoxic preconditioning-induced hypoxia-induced factor-1 alpha and Bcl-xL
YC-1, 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole, was provided by Prof. C. M. Teng, Department of Pharmacology, College of Medicine, National Taiwan University. YC-1 was developed for inhibiting HIF-1 activity in vitro and in vivo. We examined the effects of YC-1 on HP-enhanced Bcl-xL protein levels. We pretreated the HIF-1α inhibitor YC-1 (3.2 μM) intraperitoneally (i.p) before HP induction.
Induction of liver ischemia/reperfusion injury
We followed a surgical procedure as described previously. The rats were anesthetized with urethane (50 mg/kg, i.p.) and were tracheotomized. We catheterized one PE50 tubing to the femoral arterial for arterial blood pressure recording and blood sampling. One PE50 tubing was introduced to induce ischemia in the liver; the portal vein was clamped for 45 min with a small vascular clamp. Sham-operated animals underwent similar operative procedures, without occlusion of the portal vein. Reperfusion was done by removing the clamp for 6 h. After experiment, these rats were sacrificed with intravenous KCl and the blood was collected. The livers were removed and washed with cold saline. One part was stored in 10% formalin and the other was frozen in liquid nitrogen and stored at −70°C.
Determination of myeloperoxidase activity, plasma alanine aminotransferase, and aspartate aminotransferase
The liver samples were used to assay the myeloperoxidase (MPO) activity by the use of H2O2 and o-dianisidine as described previously. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were evaluated using commercial kits (Sigma, St. Louis, MO, USA).
Mitochondrial Bcl-xL protein downregulation and mitochondrial leakage of cytochrome C to cytosol are important indexes for triggering apoptotic pathway. Total homogenized proteins from livers were subjected to differential centrifugation to obtain the mitochondrial and cytosolic fractions. Protein concentration was determined by a BioRad protein assay (BioRad Laboratories). Ten micrograms of protein was electrophoresed as described below. The primary antibody of polyclonal rabbit antihuman cytochrome C (Santa Cruz), mouse antihuman Bcl-xL (Santa Cruz), goat polyclonal heat shock protein 60 (HSP60, Santa Cruz), and β-actin (Sigma) was used.
Nuclear extracts from rat livers were prepared as described previously. The antibodies raised against HIF-1α (BD Biosciences), Bad (Chemicon), and Bcl-xL and the activation fragments (32 kD of proenzyme and 17 kD of cleaved product) of caspase 3 (Upstate Biotechnology), poly-(ADP-ribose)-polymerase (PARP) (Promega), MnSOD and CuZnSOD (Enzo Life Sciences), catalase (Calbiochem), and β-actin (Sigma) were used. The density of the band with the appropriate molecular mass was determined semiquantitatively by densitometry using an image analyzing system (Alpha Innotech).
In vivo hepatic reactive oxygen species and in vitro bile reactive oxygen species determination
The ROS generation in response to hepatic I/R injury was determined from the liver surface in vivo and bile in vitro by our developed method, as described in detail previously. The bile was collected from one PE-50 tubing in the bile duct. The measurement of bile ROS was also detected by a lucigenin-amplified method, as previously described. The ROS signal was monitored continuously for an additional 300 s. The total amount of lucigenin ROS chemiluminescence was calculated by integrating of the area under the curve and subtracting it from the background level.
In situ demonstration of nitroblue tetrazolium, 3-nitrotyrosine, ED-1, and apoptosis
A nitroblue tetrazolium (NBT) perfusion method was used for localizing de novo ROS generation in the liver. The value of blue NBT deposits/total section area was counted by Adobe Photoshop 7.0.1 image software analysis.
We suggest that I/R injury might increase hepatic oxidative stress, monocyte/macrophage (Kupffer cells) infiltration, and apoptotic cellular death in the liver. We evaluated the level of oxidative stress by 3-nitrotyrosine (3-NT) accumulation,, Kupffer cell infiltration, and apoptosis formation in the paraffin-embedded sections of hepatic tissues with immunocytochemistry. The hepatic sections were deparaffinized, rehydrated, and stained immunohistochemically for the presence of 3-NT (Alpha Diagnostic International) diluted at 1:50. The percentage of 3-NT was calculated as 3-NT-stained area/total area ×100% and analyzed by Adobe Photoshop 7.0.1 image software analysis.
The method for the terminal deoxynucleotidyl transferase-mediated nick-end labeling method (TUNEL) was performed as described previously.
For hepatic monocyte/macrophage (Kupffer cells, ED-1) staining, the tissue sections were incubated overnight at 4°C with a mouse antirat antibody to ED-1 (CD68, Serotec). A biotinylated secondary antibody (Dako) was then applied followed by streptavidin conjugated to HRP (Dako). Twenty high-power (×200) fields were randomly selected for each liver section, and the value of Kupffer cells determined by ED-1-positive cells was counted.
The analysis of DNA laddering was performed as previously described.
All these values were expressed as mean ± standard error of the mean. Differences within groups were evaluated by paired t-test. One-way analysis of variance was applied to compare differences among groups. Intergroup comparisons were analyzed with Duncan's multiple-range test. A P< 0.05 was considered statistically significant.
Ethics approval statement
All studies in this article have received the institutional review board and ethics approval.
| Results|| |
Hypoxic preconditioning evokes an increase in nuclear hypoxia-inducible factor-1 alpha protein expression in livers
We determined whether HP enhanced HIF-1α expression in the livers by exploring nuclear HIF-1α protein in the rat livers under different time schedules of HP (10%). Our results demonstrated that after 1 week of our developed HP model, HIF-1α expression was significantly upregulated and maintained at the high level during 1–3 weeks of HP in rat livers [[Figure 1]A, [Figure 1]A-1].
|Figure 1: Effect of hypoxic preconditioning on nuclear hypoxia-inducible factor-1α and total proteins of Bcl-xL, MnSOD, CuZnSOD, and catalase expression. (A) Representative graph of nuclear hypoxia-inducible factor-1α expression in response to different time frame of hypoxic preconditioning in the livers. (A-1) Statistic data of A. (B) Representative graph of total proteins of Bcl-xL, MnSOD, CuZnSOD, and catalase expression in response to different time frame of hypoxic preconditioning in the livers. The statistic data of B show in (B-1 to B-4) respectively. *P < 0.05|
Click here to view
Hypoxic preconditioning evokes an increase in antiapoptotic and antioxidant protein expression in livers
We further examined the antiapoptotic protein expression of Bcl-xL and antioxidant proteins, MnSOD, CuZnSOD, and catalase in the livers from SL and HP kidneys by Western blot. [Figure 1]B and B1 to B4 demonstrate that the expression of hepatic Bcl-xL [Figure 1]B, [[Figure 1]B-1], MnSOD [Figure 1]B, [[Figure 1]B-2], CuZnSOD [Figure 1]B, [[Figure 1]B-3], and catalase [Figure 1]B, [[Figure 1]B-4] was significantly enhanced and well maintained during 1–3 weeks of HP.
Hypoxic preconditioning enhances hypoxia-inducible factor-1 alpha-dependent Bcl-xL expression in the rat livers
As shown in [Figure 2], we determined the effect of HIF-1α inhibitor YC-1 on nuclear HIF-1α and total proteins of hepatic Bcl-xL expression in the rat livers. [Figure 2] shows that YC-1 treatment significantly depressed HIF-1α and Bcl-xL expression in response to different time schedule of HP in the livers.
|Figure 2: Effect of YC-1 on nuclear hypoxia-inducible factor-1α and total proteins of hepatic Bcl-xL expression in the rat livers. (a) One typical graph of YC-1 treatment on nuclear hypoxia-inducible factor-1α expression in response to different time frame of hypoxic preconditioning in the livers. (c) Statistic data of a. (b) One typical graph of YC-1 treatment on total proteins of Bcl-xL expression in response to different time frame of hypoxic preconditioning in the livers. (d) Statistic data of b. *P < 0.05 when compared to hypoxic preconditioning|
Click here to view
Hypoxic preconditioning decreases ischemia/reperfusion injury by attenuating liver and bile reactive oxygen species
The basal level of hepatic ROS was similar between SL and HA rats. In response to hepatic I/R injury, the level of liver ROS [Figure 3]a and [Figure 3]b and bile ROS [Figure 3]c was significantly increased after 6 h of reperfusion as compared to SL or HP control. HP treatment significantly depressed I/R-enhanced parameters.
|Figure 3: Effect of hypoxic preconditioning on liver reactive oxygen species and bile reactive oxygen species in response to 45 min of ischemia and 6 h of reperfusion injury in the rats. (a) Representative graph of liver reactive oxygen species in response to ischemia and reperfusion in the sea level and hypoxic preconditioning rats. (b) The statistic data of liver reactive oxygen species. (c) The bile reactive oxygen species data from the sea level and hypoxic preconditioning rats. *P < 0.05 when compared to control (Con). #P < 0.05 hypoxic preconditioning versus sea level group|
Click here to view
Hypoxic preconditioning decreases ischemia/reperfusion injury by enhancing liver myeloperoxidase, alanine aminotransferase, and aspartate aminotransferase levels
In response to hepatic I/R injury, the level of liver MPO activity [Figure 4]a, plasma ALT concentration [Figure 4]b, and plasma AST concentration [Figure 4]c was significantly increased after 6 h of reperfusion as compared to SL or HP control. HP treatment significantly depressed I/R-enhanced parameters.
|Figure 4: Effect of hypoxic preconditioning on liver myeloperoxidase activity and liver function after hepatic ischemia/reper fusion injury. (a) Liver myeloperoxidase activity in response to hepatic ischemia/reperfusion injury. (b) Plasma alanine aminotransferase level in response to hepatic ischemia/reperfusion injury. (c) Plasma aspartate aminotransferase level in response to hepatic ischemia/reperfusion injury. *P < 0.05 when compared to sea level control. #P < 0.05 hypoxic preconditioning + ischemia/reperfusion versus sea level + ischemia/ reperfusion group|
Click here to view
Hypoxic preconditioning efficiently inhibited ischemia/reperfusion injury-induced mitochondrial dysfunction
In [Figure 5], HP significantly increased the basal level of Bcl-xL in the total proteins and mitochondrial proteins. I/R injury significantly upregulated Bad [Figure 5]a, CPP32 [Figure 5]c, and PARP [Figure 5]d expression but decreased Bcl-xL [Figure 5]b expression in the SL rat livers. HP significantly upregulated baseline Bcl-xL expression and thus inhibited the increased level of Bad, CPP32, and PARP in the HP livers. We further determined the HP effect on mitochondrial function. HP inhibited Bad translocation by I/R injury [Figure 5]a and caused restoration of mitochondrial Bcl-xL [Figure 5]e and reduction of cytosolic cytochrome C levels [Figure 5]f.
|Figure 5: Effect of hypoxic preconditioning on ischemia/reperfusion-induced apoptosis-related proteins: Bad (a), Bcl-xL (b), CPP32 (c), and poly-(ADP-ribose)-polymerase (d) expression in the rat livers. The expression of mitochondrial Bcl-xL (m-Bcl-xL) and mitochondria control protein HSP60 (e). Cytosolic cytochrome C (c-Cyto c) expression (f). HP, hypoxic preconditioning; SL, control; I/R, ischemia/ reperfusion. *P < 0.05 when compared to sea level control. #P < 0.05 HPIR versus SLIR group|
Click here to view
Hypoxic preconditioning efficiently inhibited ischemia/reperfusion-induced oxidative stress, inflammation, and apoptosis
As shown in [Figure 6], I/R increased de novo production of oxidative stress in blue NBT deposits, brown 3-NT stain [Figure 6]a,[Figure 6]b,[Figure 6]c,[Figure 6]d, Kupffer cells infiltration [Figure 6]e,[Figure 6]f,[Figure 6]g,[Figure 6]h, and apoptosis [Figure 6]i, [Figure 6]j, [Figure 6]k, [Figure 6]l formation in the in the SL rat livers. However, these I/R-enhanced oxidative stress [Figure 6]m, Kupffer cell number [Figure 6]n, and TUNEL-positive cells [Figure 6]o were significantly depressed by HP in the HP rat livers. I/R markedly induced DNA laddering in SL rats, whereas HP efficiently inhibited I/R-induced DNA laddering [Figure 6]p.
|Figure 6: Effect of hypoxic preconditioning on ischemia/reperfusion induced de novo production of oxidative stress in nitroblue tetrazolium deposits (blue precipitate, a-d) and 3-nitrotyrosine (brown stain, a-d and statistic chart in m), Kupffer cells infiltration (e-h and statistic chart in n), and apoptosis (TUNEL stain, i-l and statistic chart in o) and DNA ladder (p) expression in the rat livers. HP, hypoxic preconditioning; SL, sea level control; I/R, ischemia/reperfusion. *P < 0.05 when compared to sea level control. #P < 0.05 hypoxic preconditioning + ischemia/reperfusion versus sea level + ischemia/reperfusion group.|
Click here to view
| Discussion|| |
HP increases survival of cardiac progenitor cells and survival and engraftment in the heart through inhibition of proapoptotic elements (cytochrome C and cleaved caspase-3), preservation of the mitochondria Bcl-2 and Bcl-xL, subsequently leading to attenuate mitochondrial damages. Several lines of evidence indicated that HP treatment increases tolerance to I/R injury insults by upregulation of Bcl-2 in the kidney and in the bladder, HSP70 in the kidney, and Bcl-xL protein expression in the brain, subsequently resulting in the reduction of apoptosis and oxidative injury. Our present data further indicated that HP significantly enhanced hepatic Bcl-xL, MnSOD, Cu/ZnSOD, and catalase expression. Our data further evidenced that by the use of YC-1, a HIF-1α inhibitor Bcl-xL enhancement, was dependent on HIF-1α activation. These data could confirm previous finding that hypoxia through HIF-1α induced Bcl-xL expression, leading to antiapoptosis. Furthermore, the upregulated Bcl-xL expression associated with the downregulated Bax expression. All these data implicated that HP evokes a systemically and wide range protection to reduce oxidative stress in several organs/tissues.
It is important to evaluate the possible application potential for future. Our previous study indicated that 4 weeks of simulated 18,000-feet (5500 m) hypoxia led to polycythemia, which causes right ventricular hypertrophy and pulmonary hypertension by an increased pulmonary vascular resistance. According to our data [Figure 1], 1 week of HP can induce a protective capability like 4 weeks in the induction of HIF-1α, Bcl-xL, MnSOD, Cu/ZnSOD, and catalase. We suggest that 1 week of HP induction is enough to induce HIF-1α-related defensive mechanism in the liver and can avoid the possible adverse effect on pulmonary vasoconstriction and right ventricular hypertrophy. Cumulated evidence from basic researches also demonstrated that the HP technique can afford protective potential against oxidative injury to the kidney,, urinary bladder, brain, and cardiac progenitor cells. It is also possible that the application of selective HIF-1α inducer may be available to induce several antioxidant and antiapoptotic proteins in the target organ against I/R injury during surgery.
Augmented mitochondrial Bcl-2 or Bcl-xL protein expression by intrarenal adenoviral bcl-2 or bcl-xL gene transfer protects the kidney cells from I/R injury via the antioxidant and antiapoptotic actions. In the present study, we found that HP through a hypobaric hypoxia enhanced hepatic HIF-1α protein expression, which subsequently increased hepatic and mitochondrial Bcl-xL expression in the livers. Our data further informed that the upregulation of mitochondrial Bcl-xL inhibited cytosolic Bad translocation to mitochondria and mitochondrial cytochrome C release to cytosol in response to I/R injury. Upregulation in the HIF-1α/Bcl-xL by HP decreases cytosolic Bad translocation and mitochondrial cytochrome C release and attenuates ROS production in I/R injury liver.
HIF-1 plays a central physiological role in oxygen and energy homeostasis and is activated during hypoxia by stabilization of the subunit HIF-1α. The PI3K/Akt pathway was required for hypoxia-induced expression of HIF-1α and VEGF, whereas the MEK/ERK pathway was required only for VEGF in laser-induced rat choroidal neovascularization. Our previous study reported that HP would enhance HIF-1α-dependent HSP70 in the renal tubular cells and kidney and this upregulated HSP70 protected the kidney against renal I/R injury. Hypoxia induces a group of physiologically important genes that include erythropoietin and VEGF. Osada et al. found that a flavoprotein, NADPH-P450 reductase located at the plasma membrane, could induce erythropoietin mRNA under hypoxic conditions. Cycling hypoxia induced Bcl-xL expression via the ROS-mediated HIF-1α mechanism, leading to antiapoptosis and chemoresistance, and the use of Bcl-xL knockdown and Tempol treatment inhibited hypoxia-induced chemoresistance. Two clinical trials with 68 subjects reported ischemic preconditioning conferred protection after hepatic I/R injury based on the higher level of HIF-1α in ischemic preconditioning group compared with control group. Ischemic preconditioning in clinical trials also significantly improved biochemical markers of liver function and HIF-1α and reduced the need for reoperation in the postoperative period in the donor liver transplantation. As far we know, the technique of HP has not yet been done in the clinical trials. Luks et al. reported that to improve the quality of healthcare, people with chronic medical conditions are experiencing better quality of life and increasingly participating in a wider capacity for activities, including travel to high altitude or hypoxic exposure. In their review, the liver transplant (liver I/R) patients may tolerate high altitude (hypoxia) exposure without difficulty. It should be determined carefully the duration, the frequency, and the degree of hypoxia in future.
In recent studies, it is indicated that there are two subtypes of macrophages in liver, Kupffer cells, and monocyte-derived macrophages (MDMs). Kupffer cells belong to yolk-sac derived resident macrophage, and MDMs differentiate from bone marrow-derived or circulating monocytes. Under homeostatic conditions and liver injury, Kupffer cells are refilled by local proliferation of mature Kupffer cells. However, while Kupffer cells depletion, MDMs are recruited to the Kupffer cell niche and differentiate into Kupffer cells.,, It has been reported that Kupffer cells and MDMs express distinct transcriptomes., Moreover, it reported recently that Kupffer cells and MDMs possess differential sensitivity to bacterial LPS. While response to LPS, MDMs express more TNFα, Cxcl1, and Cxcl2 than Kupffer cells do. It indicates that MDMs have more proinflammatory phenotypes. The markers in Kupffer cells are F4/80high CD11blow Cx3crllow whereas F4/80low CD11bhigh Cx3crlhigh in MDMS. In this study, we detected Kupffer cell infiltration with monoclonal antibody, ED-1, to against CD68. CD68 is a pan marker for the various macrophage lineages, including monocytes, Kupffer cells, and osteoclasts. We found that I/R injury increased CD68-positive cells [Figure 6]g and [Figure 6]n while it significantly depressed by HP [Figure 6]h and 6n] in livers. We considered these CD68-positive cells as resident Kupffer cells; however, the proper subtypes of these CD68-positive cells need to be further elucidated.
| Conclusion|| |
In summary, I/R injury is frequently occurred during hepatic surgical procedures. Our results show that HP via the upregulation of HIF-1α-dependent Bcl-xL protein protects the liver against subsequent I/R injury by inhibition of oxidative stress, inflammation, and apoptosis. HP through pAkt/pERK-mediated pathways upregulates HIF-1α-dependent Bcl-xL protein expression in the livers. The upregulation of mitochondrial Bcl-xL could inhibit I/R-induced mitochondrial Bad and cytosolic cytochrome C translocation and subsequently reduce oxidative stress, inflammation, and apoptosis in the I/R livers.
Financial support and sponsorship
This work was supported in part by the National Science Council of the Republic of China (NSC96-2320-B-002-007 and NSC96-2221-E-002-256-MY3).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Bernaudin M, Nedelec AS, Divoux D, MacKenzie ET, Petit E, Schumann-Bard P. Normobaric hypoxia induces tolerance to focal permanent cerebral ischemia in association with an increased expression of hypoxia-inducible factor-1 and its target genes, erythropoietin and VEGF, in the adult mouse brain. J Cereb Blood Flow Metab 2002;22:393-403.
Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: A delay of lethal cell injury in ischemic myocardium. Circulation 1986;74:1124-36.
Sorimachi T, Nowak TS Jr. Pharmacological manipulations of ATP-dependent potassium channels and adenosine A1 receptors do not impact hippocampal ischemic preconditioning in vivo
: Evidence in a highly quantitative gerbil model. J Cereb Blood Flow Metab 2004;24:556-63.
Mocanu MM, Steare SE, Evans MC, Nugent JH, Yellon DM. Heat stress attenuates free radical release in the isolated perfused rat heart. Free Radic Biol Med 1993;15:459-63.
Hausenloy D, Wynne A, Duchen M, Yellon D. Transient mitochondrial permeability transition pore opening mediates preconditioning-induced protection. Circulation 2004;109:1714-7.
Ostadal B, Ostadalova I, Dhalla NS. Development of cardiac sensitivity to oxygen deficiency: Comparative and ontogenetic aspects. Physiol Rev 1999;79:635-59.
Uchiyama T, Engelman RM, Maulik N, Das DK. Role of akt signaling in mitochondrial survival pathway triggered by hypoxic preconditioning. Circulation 2004;109:3042-9.
Yellon DM, Downey JM. Preconditioning the myocardium: From cellular physiology to clinical cardiology. Physiol Rev 2003;83:1113-51.
Bolli R. The late phase of preconditioning. Circ Res 2000;87:972-83.
Depre C, Wang L, Sui X, Qiu H, Hong C, Hedhli N, et al
. H11 kinase prevents myocardial infarction by preemptive preconditioning of the heart. Circ Res 2006;98:280-8.
Rafiee P, Shi Y, Kong X, Pritchard KA Jr., Tweddell JS, Litwin SB, et al
. Activation of protein kinases in chronically hypoxic infant human and rabbit hearts: Role in cardioprotection. Circulation 2002;106:239-45.
Williams RS, Benjamin IJ. Protective responses in the ischemic myocardium. J Clin Invest 2000;106:813-8.
Yeh CH, Hsu SP, Yang CC, Chien CT, Wang NP. Hypoxic preconditioning reinforces HIF-alpha-dependent HSP70 signaling to reduce ischemic renal failure-induced renal tubular apoptosis and autophagy. Life Sci 2010;86:115-23.
Chien CT, Chang TC, Tsai CY, Shyue SK, Lai MK. Adenovirus-mediated bcl-2 gene transfer inhibits renal ischemia/reperfusion induced tubular oxidative stress and apoptosis. Am J Transplant 2005;5:1194-203.
Kim SC, Byun SH, Yang CH, Kim CY, Kim JW, Kim SG. Cytoprotective effects of Glycyrrhizae radix extract and its active component liquiritigenin against cadmium-induced toxicity (effects on bad translocation and cytochrome c-mediated PARP cleavage). Toxicology 2004;197:239-51.
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, et al
. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89.
Matsumori Y, Northington FJ, Hong SM, Kayama T, Sheldon RA, Vexler ZS, et al
. Reduction of caspase-8 and -9 cleavage is associated with increased c-FLIP and increased binding of Apaf-1 and Hsp70 after neonatal hypoxic/ischemic injury in mice overexpressing Hsp70. Stroke 2006;37:507-12.
Wang GL, Jiang BH, Rue EA, Semenza GL. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2
tension. Proc Natl Acad Sci U S A 1995;92:5510-4.
Hu S, Yan G, Xu H, He W, Liu Z, Ma G. Hypoxic preconditioning increases survival of cardiac progenitor cells via the pim-1 kinase-mediated anti-apoptotic effect. Circ J 2014;78:724-31.
Date T, Mochizuki S, Belanger AJ, Yamakawa M, Luo Z, Vincent KA, et al
. Expression of constitutively stable hybrid hypoxia-inducible factor-1alpha protects cultured rat cardiomyocytes against simulated ischemia-reperfusion injury. Am J Physiol Cell Physiol 2005;288:C314-20.
Erler JT, Cawthorne CJ, Williams KJ, Koritzinsky M, Wouters BG, Wilson C, et al
. Hypoxia-mediated down-regulation of bid and bax in tumors occurs via hypoxia-inducible factor 1-dependent and -independent mechanisms and contributes to drug resistance. Mol Cell Biol 2004;24:2875-89.
Uchida T, Rossignol F, Matthay MA, Mounier R, Couette S, Clottes E, et al
. Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: Implication of natural antisense HIF-1alpha. J Biol Chem 2004;279:14871-8.
Yang CC, Lin LC, Wu MS, Chien CT, Lai MK. Repetitive hypoxic preconditioning attenuates renal ischemia/reperfusion induced oxidative injury via upregulating HIF-1 alpha-dependent bcl-2 signaling. Transplantation 2009;88:1251-60.
Luo Y, Lu G, Chen Y, Liu F, Xu G, Yin J, et al
. Long-term cycles of hypoxia and normoxia increase the contents of liver mitochondrial DNA in rats. Eur J Appl Physiol 2013;113:223-32.
Patel A, van de Poll MC, Greve JW, Buurman WA, Fearon KC, McNally SJ, et al
. Early stress protein gene expression in a human model of ischemic preconditioning. Transplantation 2004;78:1479-87.
Kang JG, Sung HJ, Amar MJ, Pryor M, Remaley AT, Allen MD, et al
. Low ambient oxygen prevents atherosclerosis. J Mol Med (Berl) 2016;94:277-86.
Yu HJ, Chien CT, Lai YJ, Lai MK, Chen CF, Levin RM, et al
. Hypoxia preconditioning attenuates bladder overdistension-induced oxidative injury by up-regulation of Bcl-2 in the rat. J Physiol 2004;554:815-28.
Yu HJ, Lin BR, Lee HS, Shun CT, Yang CC, Lai TY, et al
. Sympathetic vesicovascular reflex induced by acute urinary retention evokes proinflammatory and proapoptotic injury in rat liver. Am J Physiol Renal Physiol 2005;288:F1005-14.
Patel N, Joseph C, Corcoran GB, Ray SD. Silymarin modulates doxorubicin-induced oxidative stress, Bcl-xL and p53 expression while preventing apoptotic and necrotic cell death in the liver. Toxicol Appl Pharmacol 2010;245:143-52.
Burgueño AL, Gianotti TF, Mansilla NG, Pirola CJ, Sookoian S. Cardiovascular disease is associated with high-fat-diet-induced liver damage and up-regulation of the hepatic expression of hypoxia-inducible factor 1α in a rat model. Clin Sci (Lond) 2013;124:53-63.
Chien CT, Lee PH, Chen CF, Ma MC, Lai MK, Hsu SM. De novo
demonstration and co-localization of free-radical production and apoptosis formation in rat kidney subjected to ischemia/reperfusion. J Am Soc Nephrol 2001;12:973-82.
Chien CT, Shyue SK, Lai MK. Bcl-xL augmentation potentially reduces ischemia/reperfusion induced proximal and distal tubular apoptosis and autophagy. Transplantation 2007;84:1183-90.
Yang XM, Wang YS, Zhang J, Li Y, Xu JF, Zhu J, et al
. Role of PI3K/Akt and MEK/ERK in mediating hypoxia-induced expression of HIF-1alpha and VEGF in laser-induced rat choroidal neovascularization. Invest Ophthalmol Vis Sci 2009;50:1873-9.
Zhao L, Liu X, Liang J, Han S, Wang Y, Yin Y, et al
. Phosphorylation of p38 MAPK mediates hypoxic preconditioning-induced neuroprotection against cerebral ischemic injury via mitochondria translocation of Bcl-xL in mice. Brain Res 2013;1503:78-88.
Chen WL, Wang CC, Lin YJ, Wu CP, Hsieh CH. Cycling hypoxia induces chemoresistance through the activation of reactive oxygen species-mediated B-cell lymphoma extra-long pathway in glioblastoma multiforme. J Transl Med 2015;13:389.
Chen CF, Chien CT, Fang HS, Chiu IS. Effects of atrial natriuretic factor in chronic hypoxic spontaneously hypertensive rats. Hypertension 1991;18:355-9.
Osada M, Imaoka S, Sugimoto T, Hiroi T, Funae Y. NADPH-cytochrome P-450 reductase in the plasma membrane modulates the activation of hypoxia-inducible factor 1. J Biol Chem 2002;277:23367-73.
Guo Y, Feng L, Zhou Y, Sheng J, Long D, Li S, et al
. Systematic review with meta-analysis: HIF-1α attenuates liver ischemia-reperfusion injury. Transplant Rev (Orlando) 2015;29:127-34.
Amador A, Grande L, Martí J, Deulofeu R, Miquel R, Solá A, et al
. Ischemic pre-conditioning in deceased donor liver transplantation: A prospective randomized clinical trial. Am J Transplant 2007;7:2180-9.
Luks AM, Swenson ER. Evaluating the risks of high altitude travel in chronic liver disease patients. High Alt Med Biol 2015;16:80-8.
Zigmond E, Samia-Grinberg S, Pasmanik-Chor M, Brazowski E, Shibolet O, Halpern Z, et al
. Infiltrating monocyte-derived macrophages and resident kupffer cells display different ontogeny and functions in acute liver injury. J Immunol 2014;193:344-53.
Yona S, Kim KW, Wolf Y, Mildner A, Varol D, Breker M, et al
. Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis. Immunity 2013;38:79-91.
Scott CL, Zheng F, De Baetselier P, Martens L, Saeys Y, De Prijck S, et al
. Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells. Nat Commun 2016;7:10321.
David BA, Rezende RM, Antunes MM, Santos MM, Freitas-Lopes MA, Diniz AB, et al
. Combination of mass cytometry and imaging analysis reveals origin, location, and functional repopulation of liver myeloid cells in mice. Gastroenterology 2016;151:1176-91.
Beattie L, Sawtell A, Mann J, Frame TCM, Teal B, de Labastida Rivera F, et al
. Bone marrow-derived and resident liver macrophages display unique transcriptomic signatures but similar biological functions. J Hepatol 2016;65:758-68.
Roth K, Rockwell CE, Copple BL. Differential sensitivity of kupffer cells and hepatic monocyte-derived macrophages to bacterial lipopolysaccharide. Clin Exp Gastroenterol Hepatol 2019;1:106.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]