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  Indian J Med Microbiol
 

Figure 1: Brain-derived neurotrophic factor increased serum response element-mediated reporter expression, CK2 enzyme activity, and serum response factor phosphorylation. PC12 cells (1.5 × 105/cm2) were transfected with pGL2-5x serum response element-SV40pr plasmid (0.5 μg) for 48 h, followed by 10 ng/mL of brain-derived neurotrophic factor treatment for 6 h. Cells were harvested for (a) a luciferase activity assay by using the Dual-Glo Luciferase Assay System or cells were lysed for a Western blot analysis of (b) CK2, (d) serum response factor phosphorylation at Ser99, and (c) a CK2 enzyme activity assay by using the CycLex CK2 Kinase Assay/Inhibitor Screening Kit (n = 9 in each group from three independent batches of cultures). Data are expressed as mean ± standard deviation. Statistical significance was evaluated using Student's t-test. ***P < 0.001

Figure 1: Brain-derived neurotrophic factor increased serum response element-mediated reporter expression, CK2 enzyme activity, and serum response factor phosphorylation. PC12 cells (1.5 × 10<sup>5</sup>/cm<sup>2</sup>) were transfected with pGL2-5x serum response element-SV40pr plasmid (0.5 μg) for 48 h, followed by 10 ng/mL of brain-derived neurotrophic factor treatment for 6 h. Cells were harvested for (a) a luciferase activity assay by using the Dual-Glo Luciferase Assay System or cells were lysed for a Western blot analysis of (b) CK2, (d) serum response factor phosphorylation at Ser99, and (c) a CK2 enzyme activity assay by using the CycLex CK2 Kinase Assay/Inhibitor Screening Kit (<i>n</i> = 9 in each group from three independent batches of cultures). Data are expressed as mean ± standard deviation. Statistical significance was evaluated using Student's <i>t</i>-test. ***<i>P</i> < 0.001