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  Indian J Med Microbiol
 

Figure 2: Knockdown of CK2α decreased serum response element-mediated reporter expression, serum response factor phosphorylation, and Mcl-1 mRNA expression. PC12 cells (2 × 105/cm2) were co-transfected with pGL2-5x serum response elemen-SV40pr plasmid (0.5 μg) and CK2α siRNA (16 pmole) for 48 h and were lysed for a Western blot analysis of (a) CK2, (c) serum response factor phosphorylation at Ser99, and (d) Mcl-1 mRNA using real-time quantitative polymerase chain reaction or cells were harvested for (b) a luciferase activity assay by using the Dual-Glo Luciferase Assay System or cells (n = 7–11 in each group from three independent batches of cultures). Data are expressed as mean ± standard deviation. Statistical significance was evaluated using Student's t test. ***P < 0.001

Figure 2: Knockdown of CK2α decreased serum response element-mediated reporter expression, serum response factor phosphorylation, and <i>Mcl-1</i> mRNA expression. PC12 cells (2 × 10<sup>5</sup>/cm<sup>2</sup>) were co-transfected with pGL2-5x serum response elemen-SV40pr plasmid (0.5 μg) and CK2α siRNA (16 pmole) for 48 h and were lysed for a Western blot analysis of (a) CK2, (c) serum response factor phosphorylation at Ser99, and (d) <i>Mcl-1</i> mRNA using real-time quantitative polymerase chain reaction or cells were harvested for (b) a luciferase activity assay by using the Dual-Glo Luciferase Assay System or cells (<i>n</i> = 7–11 in each group from three independent batches of cultures). Data are expressed as mean ± standard deviation. Statistical significance was evaluated using Student's <i>t</i> test. ***<i>P</i> < 0.001