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  Indian J Med Microbiol
 

Figure 4: Blockade of CK2α suppressed the protective effects of brain-derived neurotrophic factor on serum response element-mediated reporter expression, Mcl-1 mRNA expression, and cell viability. PC12 cells (2 × 105/cm2) were transfected with 16 pmole of CK2α siRNA for 48 h, followed by 10 ng/mL of brain-derived neurotrophic factor treatment for 6 h. The cells then received 500 nM of rotenone treatment for 24 h. The cells were harvested for (a) a luciferase activity assay by using the Dual-Glo Luciferase Assay System, (b) Mcl-1 mRNA by using real-time quantitative polymerase chain reaction, (c) cell viability determination by using an 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (n = 9 in each group from three independent batches of cultures). (d) The protein profile of Akt phosphorylation at Ser473 by the Western blot analysis. Data are expressed as mean ± standard deviation. Statistical significance was evaluated using a one-way ANOVA followed by the Newman–Kuel method. ***P < 0.001

Figure 4: Blockade of CK2α suppressed the protective effects of brain-derived neurotrophic factor on serum response element-mediated reporter expression, <i>Mcl-1</i> mRNA expression, and cell viability. PC12 cells (2 × 10<sup>5</sup>/cm<sup>2</sup>) were transfected with 16 pmole of CK2α siRNA for 48 h, followed by 10 ng/mL of brain-derived neurotrophic factor treatment for 6 h. The cells then received 500 nM of rotenone treatment for 24 h. The cells were harvested for (a) a luciferase activity assay by using the Dual-Glo Luciferase Assay System, (b) <i>Mcl-1</i> mRNA by using real-time quantitative polymerase chain reaction, (c) cell viability determination by using an 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (<i>n</i> = 9 in each group from three independent batches of cultures). (d) The protein profile of Akt phosphorylation at Ser473 by the Western blot analysis. Data are expressed as mean ± standard deviation. Statistical significance was evaluated using a one-way ANOVA followed by the Newman–Kuel method. ***<i>P</i> < 0.001