Figure 3: Record of sweat glands activated by stimulation (10% ACh, 2 mA for 5 min). Activated sweat gland density of axon reflex (indirect activated sweating by muscarinic receptor-mediated sweat activity) induced by stimulation. Activated sweat gland density of DIR (direct activated sweating by muscarinic receptor-mediated sweat activity) induced by stimulation. DIR occurs right before axon reflex. In the area between the blue and orange circles marked on the iodine-impregnated paper, sweat glands were activated by DIR. The red squared (0.5 cm x 0.5 cm) region indicates the sample area where DIR sudomotor activity were counted. The sweat glands will later be activated by axon reflex in the entire area inside the blue circle. But in the figure, the axon reflex is displayed inside the orange circle where it occurred exclusively. ACh: Acetylcholine.