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  Indian J Med Microbiol
 

Figure 2: Colorectal cancer-derived conditioned medium and glucose decreased the levels of pro-inflammatory cytokines in lipopolysaccharideactivated macrophages. Differentiated THP-1 macrophages cultured with standard medium or Caco-2-derived conditioned medium were activated with 100 ng/ml lipopolysaccharide for 24 h. The control (CON) group of THP-1 cells was untreated in standard medium. The THP-1 cells in standard medium groups were incubated in either glucose-free (φ) or glucose-containing (G, 25 mM) medium. The THP-1 cells in the conditioned medium groups were incubated with either glucose-free (φ) or glucose-containing (G, 25 mM) culture media obtained from Caco-2 cells. Levels of (a) tumor necrosis factor-α and (b) interleukin-6 were determined in macrophage cultures. *P < 0.05 versus respective standard medium groups; #P < 0.05 versus respective φ groups treated with lipopolysaccharide. The difference between the lipopolysaccharide and CON groups was statistically significant (P < 0.05), and the statistical labels were omitted from the figures for clarification of the results (n = 8/groups).

Figure 2: Colorectal cancer-derived conditioned medium and glucose decreased the levels of pro-inflammatory cytokines in lipopolysaccharideactivated macrophages. Differentiated THP-1 macrophages cultured with standard medium or Caco-2-derived conditioned medium were activated with 100 ng/ml lipopolysaccharide for 24 h. The control (CON) group of THP-1 cells was untreated in standard medium. The THP-1 cells in standard medium groups were incubated in either glucose-free (φ) or glucose-containing (G, 25 mM) medium. The THP-1 cells in the conditioned medium groups were incubated with either glucose-free (φ) or glucose-containing (G, 25 mM) culture media obtained from Caco-2 cells. Levels of (a) tumor necrosis factor-α and (b) interleukin-6 were determined in macrophage cultures. *<i>P</i> < 0.05 versus respective standard medium groups; <sup>#</sup><i>P</i> < 0.05 versus respective φ groups treated with lipopolysaccharide. The difference between the lipopolysaccharide and CON groups was statistically significant (<i>P</i> < 0.05), and the statistical labels were omitted from the figures for clarification of the results (<i>n</i> = 8/groups).