Figure 5: Glucose and lipopolysaccharide induced anti-inflammatory cytokine production in macrophages, whereas colorectal cancer-derived conditioned medium had no effect. Differentiated THP-1 macrophages activated with 100 ng/ml lipopolysaccharide were cultured with standard medium or Caco-2-derived conditioned medium. The control (CON) group of THP-1 cells was untreated in standard medium. THP-1 cells in standard medium groups were incubated in either glucose-free (φ) or glucose-containing (25 mM) medium. The THP-1 cells in the conditioned medium groups were incubated with either glucose-free (φ) or glucosecontaining (G, 25 mM) culture media obtained from Caco-2 cells. Levels of interleukin-10 were determined in macrophage cultures. *P < 0.05 versus CON+φ (n = 8/groups).