Figure 1: Effect of tectorigenin (a) on [Ca2+]i in fura-2-loaded cells. (b) Tectorigenin was added at 25 s. The concentration of tectorigenin was indicated. The experiments were performed in Ca2+-containing medium. Y axis is the [Ca2+]i induced by tectorigenin in Ca2+-containing medium. (c) Effect of tectorigenin on [Ca2+]i in the absence of extracellular Ca2+. Tectorigenin was added at 25 s in Ca2+-free medium. Y axis is the [Ca2+]i rise induced by tectorigenin in Ca2+-free medium. (d) Concentration-response plots of tectorigenin-induced [Ca2+]i rises in the presence or absence of extracellular Ca2+. Y axis is the percentage of the net (baseline subtracted) area under the curve (25–250 s) of the [Ca2+]i rises induced by 50 µM tectorigenin in Ca2+-containing medium. Data are mean ± stand error of the mean of three independent experiments. *P < 0.05 compared to open circles.