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  Indian J Med Microbiol
 

Figure 2: Effect of tectorigenin on Ca2+ influx by measuring Mn2+ quenching of fura-2 fluorescence. Experiments were performed in Ca2+-containing medium. MnCl2 (50 µM) was added to cells 1 min before fluorescence measurements. The y axis is fluorescence intensity (in arbitrary units) measured at the Ca2+-insensitive excitation wavelength of 360 nm and the emission wavelength of 510 nm. Trace a: Control, without tectorigenin. Trace b: Tectorigenin (50 µM) was added as indicated. Data are mean ± standard error of the mean of three independent experiments

Figure 2: Effect of tectorigenin on Ca<sup>2+</sup> influx by measuring Mn<sup>2+</sup> quenching of fura-2 fluorescence. Experiments were performed in Ca<sup>2+</sup>-containing medium. MnCl<sub>2</sub> (50 µM) was added to cells 1 min before fluorescence measurements. The y axis is fluorescence intensity (in arbitrary units) measured at the Ca<sup>2+</sup>-insensitive excitation wavelength of 360 nm and the emission wavelength of 510 nm. Trace a: Control, without tectorigenin. Trace b: Tectorigenin (50 µM) was added as indicated. Data are mean ± standard error of the mean of three independent experiments