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  Indian J Med Microbiol
 

Figure 5: Effect of U73122 on tectorigenin-induced Ca2+ release. Experiments were performed in Ca2+-free medium. (a) Adenosine triphosphate (10 μM) was added at 25 s. (b) The first column is 50 μM tectorigenin-induced [Ca2+]i rises. The second column shows that 2 μM U73122 did not alter basal [Ca2+]i. The third column shows ATP-induced [Ca2+]i rises. The fourth column shows that U73122 pretreatment for 60 s abolished ATP-induced [Ca2+]i rises (*P < 0.05 compared to 3rd column). The fifth column shows that U73122 (incubation for 60 s) and adenosine triphosphate (incubation for 30 s) pretreatment inhibited 50 μM tectorigenin-induced [Ca2+]i rises. The sixth column shows that combination of adenosine triphosphate (10 μM) tectorigenin (50 μM) did not induce [Ca2+]i rises synergistically. Data are mean ± standard error of the mean of three independent experiments

Figure 5: Effect of U73122 on tectorigenin-induced Ca<sup>2+</sup> release. Experiments were performed in Ca<sup>2+</sup>-free medium. (a) Adenosine triphosphate (10 μM) was added at 25 s. (b) The first column is 50 μM tectorigenin-induced [Ca<sup>2+</sup>]i rises. The second column shows that 2 μM U73122 did not alter basal [Ca<sup>2+</sup>]i. The third column shows ATP-induced [Ca<sup>2+</sup>]i rises. The fourth column shows that U73122 pretreatment for 60 s abolished ATP-induced [Ca<sup>2+</sup>]i rises (*<i>P</i> < 0.05 compared to 3rd column). The fifth column shows that U73122 (incubation for 60 s) and adenosine triphosphate (incubation for 30 s) pretreatment inhibited 50 μM tectorigenin-induced [Ca<sup>2+</sup>]i rises. The sixth column shows that combination of adenosine triphosphate (10 μM) tectorigenin (50 μM) did not induce [Ca<sup>2+</sup>]i rises synergistically. Data are mean ± standard error of the mean of three independent experiments