Figure 5: Microscopic images of liver tissue in different groups (4-μm-thick sections, H and E staining, magnification ×100, ×200, and × 400) and apoptosis induction following Dich and the IA3 usage (×400, TUNEL staining). Liver section in the sham group (a and f), normal liver structure. Liver section of Dich group (b, e, h, and k), increased white blood cells (inflammation) (black arrows and triangle sign), and central hepatic vein dilatation (star), the vacuolization hepatocyte (necrosis) (square sign), sinusoidal dilatation (red arrow), and hyperemia (circle), due to the oxidative stress caused by Dich. Liver section in IA group (1000 mg/kg) (c and g), normal liver structure. Micrograph of the liver section in IA + Dich (1000 mg/ kg) group (d, i and l), normal liver structure (scale bars: black = 1 μm). (j) Left: cytoplasm staining, middle: nuclei staining, right: merge. The yellow arrows refer to the shiny green nuclei of apoptotic cells. IA: Ipomoea aquatica.