• Users Online: 532
  • Print this page
  • Email this page
Year : 2020  |  Volume : 63  |  Issue : 4  |  Page : 179-186

Novel regulation of PKC-induced inflammation by Akt and protein phosphatase 2A in ovarian granulosa cells

1 Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan
2 Department of Obstetrics and Gynecology, Cheng Hsin General Hospital, Taipei, Taiwan

Correspondence Address:
Prof. Yuh-Lin Wu
Room 405, Shouren Building, National Yang-Ming University, No 155, Sec. 2, Linong Street, Taipei City 112
Login to access the Email id

Source of Support: This research was supported by grants from the Ministry of Science and Technology (MOST 105-2320-B-010-026-MY3; MOST 108-2320-B-010-025), the Cheng Hsin General Hospital (CY10603; CY10802), and the Taiwan Ministry of Education Aim for the Top University Plan., Conflict of Interest: None

DOI: 10.4103/CJP.CJP_44_20

Rights and Permissions

PKC-mediated inflammation is important in ovarian physiology. The roles of Akt and protein phosphatase 2A (PP2A) in PKC-mediated inflammation in ovarian granulosa cells (GCs) remain mostly unclear. PKC activator phorbol 12-myristate 13-acetate induced the Akt phosphorylation in rat primary GCs but reduced the Akt phosphorylation in KGN human GCs. In rat GCs, an inhibitory effect of PI3K inhibitor wortmannin and a stimulatory effect of Akt activator SC79 on PKC-induced cyclooxygenase-2 (COX-2)/PGE2production were noted; wortmannin and SC79 acted oppositely in human GCs. In rat GCs, PP2A inhibitor okadaic acid further enhanced the PKC-mediated promoter activation and elevation of mRNA and protein levels of the COX-2 gene, whereas PP2A activator sodium selenate attenuated the PKC-mediated COX-2 expression and promoter activation. PKC activation did not affect PP2A phosphorylation, but okadaic acid indeed augmented the PKC-induced NF-κB nuclear translocation. Thus, PP2A appears to act as a negative modulator in PKC-mediated cellular inflammation in rat GCs, at least in part due to its attenuating effect on the PKC-induced NF-κB activation.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded117    
    Comments [Add]    

Recommend this journal