| ORIGINAL ARTICLE |
|
| Year : 2023 | Volume
: 66
| Issue : 1 | Page : 28-35 |
|
|
Protocatechuic acid reduces H2O2-induced migration and oxidative stress of fibroblast-like synoviocytes in rheumatoid arthritis by activating Nrf2-Keap1 signaling pathway
Yan Liu1, Yucheng Zhang2, Keke Zhang3, Yue Wang1
1 Department of Rheumatology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China
2 Department of Critical Care Medicine, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Jiangsu, China
3 School of Integrated Traditional Chinese and Western Medicine, Jiangsu Health Vocational College, Nanjing, Jiangsu, China
Correspondence Address:
Dr. Yue Wang
Department of Rheumatology, Affiliated Hospital of Nanjing University of Chinese Medicine, No. 155, Hanzhong Road, Nanjing, 210029, Jiangsu
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/cjop.CJOP-D-22-00087
|
|
|
Honeycomb (Nidus vespae) is traditional Chinese medicine and can treat rheumatoid arthritis (RA), and protocatechuic acid (PCA) is a bioactive component of honeycomb. This study aimed to investigate whether PCA could reduce the H2O2-induced migration and oxidative stress of RA fibroblast-like synoviocytes (RA-FLSs). H2O2-induced RA-FLSs were used to simulate the in vitro model of RA. The viability, apoptosis, migration, invasion, and oxidative stress of RA-FLSs were detected by Cell Counting Kit-8 (CCK-8), terminal deoxynucleotidyl transferase dUTP nick-end labeling assay, wound healing, transwell assays, DCFDA staining, and malonaldehyde and superoxide dismutase enzyme-linked immunosorbent assay kits. The expression of migration and invasion-related proteins and Nrf2/Keap1 signaling pathway-related proteins was analyzed by western blotting. As a result, PCA suppressed the viability, migration, invasion, and oxidative and promoted apoptosis of H2O2-induced RA-FLSs by activating the Nrf2/Keap1 signaling pathway. ML-385, an Nrf2 inhibitor, could enhance the viability, migration, invasion, and oxidative and inhibited apoptosis of H2O2-induced RA-FLSs. In conclusion, PCA reduced H2O2-induced migration and oxidative stress of RA-FLSs by activating the Nrf2-Keap1 signaling pathway.
|
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
