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  Indian J Med Microbiol
 

Figure 1: Analysis of tumor size, body mass, tumor-free body mass, grip strength and epididymal fat mass in mice. Analyses of BALB/c mice injected subcutaneously with CT-26 cells inducing cachexia, or with PBS. Intraperitoneal injection of PBS, acylated ghrelin or unacylated ghrelin to the CT-26-tumor-bearing mice were performed. Tumor volume (a) and body mass (b) were recorded daily for 14 days, no significant differences were detected among the four groups, by one-way ANOVA followed by Tukey test. On day 28, the tumor was surgically excised and weighed, allowing calculation of the percentage change in tumor-free body mass from pre-inoculation (c); significant differences were detected between NC and T + V groups, by one-way ANOVA followed by Tukey test, F = 18.056, P < 0.001. Grip strength was assessed on day 1 before tumor implantation and day 28, allowing calculation of the percentage change in grip strength from pre-inoculation (d); significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 50.884, P < 0.001. The epididymal fat was excised and weighed on an analytical balance (e); significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Dunnett T3 test, F = 443.651, P < 0.001 (*P < 0.05, **P < 0.01, ***P < 0.001).

Figure 1:  Analysis of tumor size, body mass, tumor-free body mass, grip strength and epididymal fat mass in mice. Analyses of BALB/c mice injected subcutaneously with CT-26 cells inducing cachexia, or with PBS. Intraperitoneal injection of PBS, acylated ghrelin or unacylated ghrelin to the CT-26-tumor-bearing mice were performed. Tumor volume (a) and body mass (b) were recorded daily for 14 days, no significant differences were detected among the four groups, by one-way ANOVA followed by Tukey test. On day 28, the tumor was surgically excised and weighed, allowing calculation of the percentage change in tumor-free body mass from pre-inoculation (c); significant differences were detected between NC and T + V groups, by one-way ANOVA followed by Tukey test, F = 18.056, <i>P</i> < 0.001. Grip strength was assessed on day 1 before tumor implantation and day 28, allowing calculation of the percentage change in grip strength from pre-inoculation (d); significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 50.884, <i>P</i> < 0.001. The epididymal fat was excised and weighed on an analytical balance (e); significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Dunnett T3 test, F = 443.651, <i>P</i> < 0.001 (*<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001).