Figure 2: Effect of AG and UnAG on lower limb muscles and tumors. (a) Macroscopic view of tumor-free mice and tumor-bearing mice at the end of the experiment. (b) Macroscopic view of tumors and lower limbs excised from mice at the end of the experiment. (c) Immunofluorescence staining images of a middle cross-section of the midbelly region of gastrocnemii incubated with anti-MHC antibody (×100). Bar represents 50 μm. (d) The average CSA of myofibers in the gastrocnemii; significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Dunnett T3 test, F = 46.812, P < 0.001. (*P < 0.05, **P < 0.01, ***P < 0.001).