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  Indian J Med Microbiol
 

Figure 3: Regulation of calpain system activity by tumor-bearing and AG/UnAG administration in gastrocnemii of mice. (a) Western blot of protein levels. (b) Quantification of calpain-1 was normalized to GAPDH. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 31.361, P < 0.001. (c) Quantification of calpain-2 was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (d) Quantification of calpain-3 was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (e) Quantification of calpastatin was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (f) Quantification of calpain-1/calpastatin ratio. Significant differences were detected between T + V and NC groups, between T + V and AG groups, by one-way ANOVA followed by Dunnett T3 test, F = 29.918, P < 0.001. (g) Quantification of calpain activity. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 23.979, P = 0.001. Data are represented as mean ± standard deviation (*P < 0.05, **P < 0.01, ***P < 0.001).

Figure 3:  Regulation of calpain system activity by tumor-bearing and AG/UnAG administration in gastrocnemii of mice. (a) Western blot of protein levels. (b) Quantification of calpain-1 was normalized to GAPDH. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 31.361, <i>P</i> < 0.001. (c) Quantification of calpain-2 was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (d) Quantification of calpain-3 was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (e) Quantification of calpastatin was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (f) Quantification of calpain-1/calpastatin ratio. Significant differences were detected between T + V and NC groups, between T + V and AG groups, by one-way ANOVA followed by Dunnett T3 test, F = 29.918, <i>P</i> < 0.001. (g) Quantification of calpain activity. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 23.979, <i> P </i>= 0.001. Data are represented as mean ± standard deviation (*<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001).