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  Indian J Med Microbiol
 

Figure 4: Regulation of ubiquitin E3 legases expression and Akt/mTOR pathway by tumor-bearing and AG/UnAG administration in gastrocnemii of mice. (a) Western blot of protein levels. (b) Quantification of atrogin-1 was normalized to GAPDH. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 23.981, P < 0.001. (c) Quantification of MuRF1 was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (d) Quantification of p-Akt was normalized to Akt. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 17.941, P = 0.001. (e) Quantification of p-mTOR was normalized to mTOR. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. Data are represented as mean ± standard deviation (*P < 0.05, **P < 0.01, ***P < 0.001).

Figure 4: Regulation of ubiquitin E3 legases expression and Akt/mTOR pathway by tumor-bearing and AG/UnAG administration in gastrocnemii of mice. (a) Western blot of protein levels. (b) Quantification of atrogin-1 was normalized to GAPDH. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 23.981, <i>P</i> < 0.001. (c) Quantification of MuRF1 was normalized to GAPDH. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. (d) Quantification of p-Akt was normalized to Akt. Significant differences were detected between T + V and NC groups, between T + V and AG/UnAG groups, by one-way ANOVA followed by Tukey test, F = 17.941, <i>P</i> = 0.001. (e) Quantification of p-mTOR was normalized to mTOR. No significant differences were detected between four groups, by one-way ANOVA followed by Tukey test. Data are represented as mean ± standard deviation (*<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001).