Figure 2: Sympathetic innervation in the spleen induces T-lymphocyte activation in HFD offspring. (a) Representative photomicrographs showing the catecholaminergic containing fibers (arrows), visualized using the sucrose-potassium phosphate-glyoxylic acid method, in the spleen of ND, HFD and HFD offspring subjected to SND performed at 5 weeks of age. (b) Representative HPLC chromatography and group data showing tissue NE levels, and (c) percentage of CD4+ and CD8+ cells within the CD3+ cell population in the spleen of ND, HFD and HFD + SND at 6 weeks of age. Data are presented as mean ± SEM, n = 6 per group in b and c. *P < 0.05 versus ND group and #P < 0.05 versus HFD group in post-hoc Tukey's multiple range analysis. SND: Sympathetic nerve denervation, HFD: High fructose diet, ND: Normal diet, SEM: Standard error of mean, HPLC: High-performance liquid chromatography, NE: Norepinephrine.