Figure 5: Redox-dependent activation of T-lymphocytes in the spleen of HFD offspring. (a) Tissue ROS and NE levels, (b) percentage of CD4⁺ and CD8⁺ cells within the CD3⁺ cell population in the spleen, and (c) SBP of ND or HFD offspring at 9 weeks of age that received tempol treatment in the drinking water (1 mmol/L) for 1 week at 6 weeks of age. Data are presented as mean ± SEM, n = 5–6 per group at each age. *P < 0.05 versus ND group and ^#P < 0.05 versus HFD group in post hoc Tukey's multiple range analysis. ROS: Reactive oxygen species, ND: Normal diet, SEM: Standard error of the mean, NE: Norepinephrine, HFD: High fructose diet, SBP: Systolic blood pressure.