Figure 2: Anti-apoptosis and anti-necrosis effects of Astragalus-containing serum. PC12 cells were cultured with 10% blended serum containing 8% Astragalus-containing serum and 2% control serum for 1 h and then added 100 μM 6-hydroxydopamine for another 24 h. (a-i) After Hoechst 33342/propidium iodide staining, fluorescent images were capture by virtue of fluorescence microscope. PC12 cells which display dull blue fluorescence indicate viable cells, whereas the apoptotic cells display bright blue fluorescence. Red fluorescence represents dead cells. (j and k) Apoptosis and necrosis of cultured cells were detected. The percentage of apoptotic cells and necrotic cells was significantly decreased when co-incubated with Astragalus-containing serum. **P < 0.01 (j and k, vs. 10% control serum + 100 μM 6-hydroxydopamine group); ***P < 0.001 (j and k, vs. 10% control serum group). White arrows indicate apoptotic cell, and yellow arrows indicate necrotic cell. The scale bar in a indicates 100 μm (n = 3).