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  Indian J Med Microbiol
 

Figure 3: Apoptotic rate determined by flow cytometry. PC12 cells were cultured with 10% control serum (a and b) and 8% Astragalus-containing serum + 2% control serum (c) for 1 h, respectively, and then added 100 μM 6-hydroxydopamine to b and c group for another 24 h; then, flow cytometry was used for the detection of the apoptosis rate (a-c). The early apoptosis rate of cells was significantly decreased in the Astragalus-containing serum group (d). ***P < 0.001 (d, vs. 10% control serum group); ##P < 0.01 (d, vs. 10% control serum + 100 μM 6-hydroxydopamine group) (n = 4).

Figure 3: Apoptotic rate determined by flow cytometry. PC12 cells were cultured with 10% control serum (a and b) and 8% <i>Astragalus</i>-containing serum + 2% control serum (c) for 1 h, respectively, and then added 100 μM 6-hydroxydopamine to b and c group for another 24 h; then, flow cytometry was used for the detection of the apoptosis rate (a-c). The early apoptosis rate of cells was significantly decreased in the <i>Astragalus</i>-containing serum group (d). ***<i>P</i> < 0.001 (d, vs. 10% control serum group); <sup>##</sup><i>P</i> < 0.01 (d, vs. 10% control serum + 100 μM 6-hydroxydopamine group) (<i>n</i> = 4).