Figure 5: Astragalus-containing serum rescued the depolarization of mitochondrial membrane potential after 6-hydroxydopamine treatment. (a-c) The mitochondrial membrane potential of cultured PC12 cells was detected using JC-1 dye. (a) The healthy cells of the control showed that yellow fluorescence indicated high mitochondrial membrane potential with aggregate of JC-1. (b) In the 6-hydroxydopamine group, some cells showed that green fluorescence indicated low mitochondrial membrane potential with JC-1 of monomer. (c) Astragalus-containing serum partly reversed the changes caused by 6-hydroxydopamine. (d) The Astragalus-containing serum protection group showed more yellow fluorescence and less green fluorescence compared to the 6-hydroxydopamine damage group. **P < 0.01 (d, vs. 10% control serum group); #P < 0.05 (d, vs. 10% control serum + 100 μM 6-hydroxydopamine group). The scale bar = 100 μm (n = 3).