Figure 2: Effect of Alpinia officinarum Hance extract on prostaglandin E2 production and cyclooxygenase-2 expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Cells were treated with or without 1 μg/mL LPS and 37.5 μg/mL Alpinia officinarum Hance extract and then incubated for 24 h. Prostaglandin E2 content in media was evaluated by ELISA (a) and cyclooxygenase-2 expression levels were evaluated by Western blot (b, upper panel). The expression level of each protein was then quantified using ImageJ software. After normalization with β-actin, relative cyclooxygenase-2 expression levels are shown (b, lower panel). NC: no-treatment control, LPS: treated with LPS only, AOE: treated with AOE only, L + A: treated with LPS and AOE. #P < 0.05 compared to NC; *P < 0.05 compared to LPS.