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  Indian J Med Microbiol
 

Figure 1: Ca2+ influx stimulated by spermine. (a) [Ca2+]i was measured in bEND.3 cells bathed in Ca2+-containing solution and cells were exposed to 3 mM spermine. (b) [Ca2+]i was measured in bEND.3 cells bathed in Ca2+-free solution. Cells were pretreated with or without 10 μM SKF 96365 for 8 min (red and black lines, respectively); after cells were stimulated by 3 mM spermine, 2 mM Ca2+ was replenished. The green line represents the results obtained when cells received no SKF 96365 pre-treatment and spermine stimulation before Ca2+ replenishment. There are significant (*P < 0.05) differences between the spermine-SKF group and spermine-no SKF group between 403 and 583 s. (c) [Ca2+]i was measured in bEND.3 cells bathed in Ca2+-containing solution and cells were treated with 30 μM cyclopiazonic acid. Spermine results obtained in Figure 1a are overlapped here for comparison. Significant (*P < 0.05) differences between the spermine and cyclopiazonic acid group began after 278 s. (d) [Ca2+]i was measured in bEND.3 cells bathed in Ca2+-free solution. After cells were treated with 30 μM cyclopiazonic acid, 2 mM Ca2+ was replenished. Spermine results obtained in Figure 1b are overlapped here for comparison. Significant (*P < 0.05) differences between the spermine and cyclopiazonic acid group began after 500 s. Results are mean ± SEM, with each group having 23–29 cells obtained from three to four separate experiments.

Figure 1: Ca<sup>2+</sup> influx stimulated by spermine. (a) [Ca<sup>2+</sup>]i was measured in bEND.3 cells bathed in Ca<sup>2+</sup>-containing solution and cells were exposed to 3 mM spermine. (b) [Ca<sup>2+</sup>]i was measured in bEND.3 cells bathed in Ca<sup>2+</sup>-free solution. Cells were pretreated with or without 10 μM SKF 96365 for 8 min (red and black lines, respectively); after cells were stimulated by 3 mM spermine, 2 mM Ca<sup>2+</sup> was replenished. The green line represents the results obtained when cells received no SKF 96365 pre-treatment and spermine stimulation before Ca<sup>2+</sup> replenishment. There are significant (*<i>P</i> < 0.05) differences between the spermine-SKF group and spermine-no SKF group between 403 and 583 s. (c) [Ca<sup>2+</sup>]i was measured in bEND.3 cells bathed in Ca<sup>2+</sup>-containing solution and cells were treated with 30 μM cyclopiazonic acid. Spermine results obtained in Figure 1a are overlapped here for comparison. Significant (*<i>P</i> < 0.05) differences between the spermine and cyclopiazonic acid group began after 278 s. (d) [Ca<sup>2+</sup>]i was measured in bEND.3 cells bathed in Ca<sup>2+</sup>-free solution. After cells were treated with 30 μM cyclopiazonic acid, 2 mM Ca<sup>2+</sup> was replenished. Spermine results obtained in Figure 1b are overlapped here for comparison. Significant (*<i>P</i> < 0.05) differences between the spermine and cyclopiazonic acid group began after 500 s. Results are mean ± SEM, with each group having 23–29 cells obtained from three to four separate experiments.