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  Indian J Med Microbiol
 

Figure 4: Suppressive effects of 2-APB and ruthenium red on Ca2+ signals triggered by high Ca2+, cinacalcet , and spermine. [Ca2+]i was measured in bEND.3 cells bathed in Ca2+-containing solution; cells were pretreated with or without 50 μM 2-APB or 30 μM ruthenium red for 8 min and then treated with (a) 3 mM Ca2+; significant (P < 0.05) differences between the control and 2-APB group and ruthenium red group began after 183 (*) and 107 s (**), respectively; (b) 10 μM cinacalcet; significant (P < 0.05) differences between the control and 2-APB group and ruthenium red group began after 115 (*) and 100 s (**), respectively; or (c) 3 mM spermine; there were significant (P < 0.05) differences between the control and 2-APB group from 102 to 318 s (*), and between the control and ruthenium red group from 122 to 340 s (**). Results are mean ± SEM, with each group having 23–41 cells obtained from three to four separate experiments.

Figure 4: Suppressive effects of 2-APB and ruthenium red on Ca<sup>2+</sup> signals triggered by high Ca<sup>2+</sup>, cinacalcet , and spermine. [Ca<sup>2+</sup>]i was measured in bEND.3 cells bathed in Ca<sup>2+</sup>-containing solution; cells were pretreated with or without 50 μM 2-APB or 30 μM ruthenium red for 8 min and then treated with (a) 3 mM Ca<sup>2+</sup>; significant (<i>P</i> < 0.05) differences between the control and 2-APB group and ruthenium red group began after 183 (*) and 107 s (**), respectively; (b) 10 μM cinacalcet; significant (<i>P</i> < 0.05) differences between the control and 2-APB group and ruthenium red group began after 115 (*) and 100 s (**), respectively; or (c) 3 mM spermine; there were significant (<i>P</i> < 0.05) differences between the control and 2-APB group from 102 to 318 s (*), and between the control and ruthenium red group from 122 to 340 s (**). Results are mean ± SEM, with each group having 23–41 cells obtained from three to four separate experiments.